Effects of proprioceptive neuromuscular facilitation combined with threshold inspiratory muscle training on respiratory function in neurocritical patients with weaning failure: a randomized controlled trial.

The purpose of this study was to determine the effects of combining proprioceptive neuromuscular facilitation (PNF) with threshold inspiratory muscle training (TIMT), compared with TIMT alone, on respiratory function in neurocritical patients who experienced a weaning failure. Forty-seven participants (mostly after a stroke), were randomly divided into the experimental group (n = 24) and the control group (n = 23). The control group received usual care and TIMT, whereas the experimental group, in addition, underwent four 90-s periods of manual PNF. Both groups performed training in the ICU twice a day for 5 consecutive days. The main outcome measures included maximum inspiratory pressure, diaphragmatic excursions, diaphragm thickening fraction, oxygenation index, and forced expiratory volume in 1 s/forced vital capacity. The results showed a significant group-by-time interaction effect for maximum inspiratory pressure [F (1, 45) = 17.84, η2 = 0.328, P < 0.001] and oxygenation index [F [1, 45) = 5.58, η2 = 0.11, P = 0.023]. When compared with the control group, the experimental group showed overall significantly higher maximum inspiratory pressure [mean difference = 4.37 cm H2O, 95% confidence interval (CI) 0.25-8.50, P = 0.038]. No other significant group differences were found. Combining PNF with TIMT may improve respiratory function in neurocritical patients with weaning failure. This combination approach may increase the likelihood of survival of neurocritical patients in the ICU.

Repetitive peripheral magnetic stimulation combined with transcranial magnetic stimulation in rehabilitation of upper extremity hemiparesis following stroke: a pilot study.

OBJECTIVE: To investigate the effect of combined repetitive peripheral magnetic stimulation and transcranial magnetic stimulation on upper extremity function in subacute stroke patients. DESIGN: Pilot study. SUBJECTS: Subacute stroke patients. METHODS: Included patients were randomized into 3 groups: a central-associated peripheral stimulation (CPS) group, a central-stimulation-only (CS) group, and a control (C) group. The CPS group underwent a new paired associative stimulation (combined repetitive peripheral magnetic stimulation and transcranial magnetic stimulation), the CS group underwent repetitive transcranial magnetic stimulation, and the C group underwent sham stimulation. All 3 groups received physiotherapy after the stimulation or sham stimulation. The treatment comprised 20 once-daily sessions. Primary outcome was the Fugl-Meyer Assessment Upper Extremity (FMA-UE) score, and secondary outcomes were the Barthel Index and Comprehensive Functional Assessment scores, and neurophysiological assessments were mainly short-interval intracortical inhibition. A 3-group (CPS, CS, C) × 2-time (before, after intervention) repeated measures analysis of variance was conducted to determine whether changes in scores were significantly different between the 3 groups. RESULTS: A total of 45 patients were included in the analysis. Between-group comparisons on the FMA-UE demonstrated a significant improvement (group × time interaction, F2,42 = 4.86; p = 0.013; C vs CS, p = 0.020; C vs CPS, p = 0.016; CS vs CPS, p = 0.955). Correlation analysis did not find any substantial positive correlation between changes in FMA-UE and short-interval intracortical inhibition variables (C, r = -0.196, p = 0.483; CS, r = -0.169, p = 0.546; CPS, r = -0.424, p = 0.115). CONCLUSION: This study suggests that the real-stimulus (CS and CPS) groups had better outcomes than the control (C) group. In addition, the CPS group showed a better trend in clinical and neurophysiological assessments compared with the CS group.

Distinct serum GDNF coupling with brain structural and functional changes underlies cognitive status in Parkinson's disease.

AIM: Aberrations in brain connections are implicated in the pathogenesis of Parkinson's disease (PD). We previously demonstrated that Glial cell-derived neurotrophic factor (GDNF) reduction is associated with cognition decline. Nonetheless, it is elusive if the pattern of brain topological connectivity differed across PD with divergent serum GDNF levels, and the accompanying profile of cognitive deficits has yet to be determined. METHODS: We collected data on the participants' cognition, demographics, and serum GDNF levels. Participants underwent 3.0T magnetic resonance imaging, and we assessed the degree centrality, brain network topology, and cortical thickness of the healthy control (HC) (n = 25), PD-high-GDNF (n = 19), and PD-low-GDNF (n = 19) groups using graph-theoretic measures of resting-state functional MRI to reveal how much brain connectivity varies and its clinical correlates, as well as to determine factors predicting the cognitive status in PD. RESULTS: The results show different network properties between groups. Degree centrality abnormalities were found in the right inferior frontal gyrus and right parietal lobe postcentral gyrus, linked with cognition scores. The two aberrant clusters serve as a potentially powerful signal for determining whether a patient has PD and the patient's cognition level after integrating with GDNF, duration, and dopamine dosage. Moreover, we found a significant positive relationship between the thickness of the left caudal middle frontal lobe and a plethora of cognitive domains. Further discriminant analysis revealed that the cortical thickness of this region could distinguish PD patients from healthy controls. The mental state evaluation will also be more precise when paired with GDNF and duration. CONCLUSION: Our findings reveal that the topological features of brain networks and cortical thickness are altered in PD patients with cognitive deficits. The above change, accompanied by the serum GDNF, may have merit as a diagnosis marker for PD and, arguably, cognition status.

Corrigendum: The After-Effect of Accelerated Intermittent Theta Burst Stimulation at Different Session Intervals.

[This corrects the article DOI: 10.3389/fnins.2020.00576.].

Plant material and its biochar differ in their effects on nitrogen mineralization and nitrification in a subtropical forest soil.

Natural wildfires have a great effect on soil N transformation in subtropical forest. The pyrogenic organic matter (PyOM) in forest soils is mainly derived from the plant material burnt during forest fires, which affects soil N composition, N mineralization and nitrification. This study examined the effects of typical fresh plant material (leaves and twigs of Castanopsis sclerophylla, representing litter) and its biochar (representing PyOM) on N mineralization and nitrification in a subtropical forest soil. The soils were incubated with the plant material (PM), its biochar (BC) and their combinations for 84 days. Both PM and BC considerably increased soil pH and dissolved organic C, whereas PM decreased NO3--N and dissolved organic N. The additions of PM alone, and its combinations with BC resulted in net N immobilization. The rates of net N mineralization rapidly increased in first 14 days and then became stable following the addition of PM to soil. Moreover, the additions of PM and BC increased the abundances of archaeal amoA and bacterial amoA, especially with PM. The abundance of bacterial amoA correlated positively with soil pH and dissolved organic C, while archaeal amoA showed the opposite. Biochar affected soil properties and N transformation more significantly in the presence of PM, highlighting the need for further research on the interactions of plant litter and its biochar.

The After-Effect of Accelerated Intermittent Theta Burst Stimulation at Different Session Intervals.

OBJECTIVE: The study aims to investigate the after-effect of three sessions of intermittent theta-burst stimulation (iTBS) on motor cortical excitability. The iTBS was induced over the primary motor cortex (M1) at different time intervals. METHODS: The study has a crossover design. Sixteen participants were assigned to three groups and received different accelerated iTBS (aiTBS) protocols during each visit: (1) three continuous sessions with no interval (iTBS18000); (2) three iTBS sessions with 10-min intervals (iTBS600 × 3∗10); and (3) three iTBS sessions with 30-min intervals (iTBS600 × 3∗30). As washout period, each visit is separated by at least 7 days. We measured the motor cortical excitability changes and intracortical inhibition. RESULTS: A dose of 1,800 pulses of aiTBS per day is tolerable. The iTBS1800 led to a reduced cortical excitability; whereas iTBS600 × 3∗10 and iTBS600 × 3∗30 enhanced cortical excitability to a differential extent. After a total dose of 1,800 pulses, iTBS600 × 3∗30 exhibited the longer effect and highest percentage of individuals with enhanced cortical excitability. CONCLUSION: The results suggest that aiTBS protocols at different time intervals result in different motor cortical excitability after-effects.

Genome assembly of Nannochloropsis oceanica provides evidence of host nucleus overthrow by the symbiont nucleus during speciation.

The species of the genus Nannochloropsis are unique in their maintenance of a nucleus-plastid continuum throughout their cell cycle, non-motility and asexual reproduction. These characteristics should have been endorsed in their gene assemblages (genomes). Here we show that N. oceanica has a genome of 29.3 Mb consisting of 32 pseudochromosomes and containing 7,330 protein-coding genes; and the host nucleus may have been overthrown by an ancient red alga symbiont nucleus during speciation through secondary endosymbiosis. In addition, N. oceanica has lost its flagella and abilities to undergo meiosis and sexual reproduction, and adopted a genome reduction strategy during speciation. We propose that N. oceanica emerged through the active fusion of a host protist and a photosynthesizing ancient red alga and the symbiont nucleus became dominant over the host nucleus while the chloroplast was wrapped by two layers of endoplasmic reticulum. Our findings evidenced an alternative speciation pathway of eukaryotes.

Chemical speciation and risk assessment of Cu and Zn in biochars derived from co-pyrolysis of pig manure with rice straw.

Pig manure has been utilized as a good feedstock to produce biochar. However, the pig manure-derived biochar from intensive pig cultivation contains high levels of total and bioavailable heavy metals. In this study, the co-pyrolysis of pig manure with other biomass (e.g. rice straw) at 300-700 °C was investigated to solve the above-mentioned topic. The ammonium acetate (CH3COONH4), Tessier sequential extraction procedure and hydrogen peroxide were adopted to evaluate the bioavailability, chemical speciation, and potential risk of Cu and Zn in the biochars. Results showed that the addition of rice straw significantly reduced the concentrations of total, exchangeable and carbonate-associated Cu and Zn in the biochars compared to the single pig manure biochars. Co-pyrolysis of pig manure with rice straw at a mass ratio of 1:3 and at 600 °C could be most effective to reduce the concentrations of CH3COONH4-extractable and potential released Cu and Zn in the biochars. In conclusion, the co-pyrolysis process is a feasible management for the safe disposal of metal-polluted pig manure in an attempt to reduce the bioavailability and potential risk of heavy metals at relatively high pyrolysis temperatures.

Tissue-overlapping response of half-smooth tongue sole (Cynoglossus semilaevis) to thermostressing based on transcriptome profiles.

Thermal stress, encountered frequently in aquaculture, affects diverse physiological processes and behavior of fish; however, the mechanism underlying these effects may vary among species. Half-smooth tongue sole (Cynoglossus semilaevis) widely inhabits Asian coastal waters and has been intensively cultured in China. In this study, the transcriptomes of three tissues (gill, liver and muscle) of half-smooth tongue sole stressed at temperatures up to 35°C were profiled and compared with those of the fish living at a normal temperature of 25°C. Of 105,228 transcripts, 23,213 were annotated in GO terms, and 718, 1236 and 561 were found to express differentially in gill, liver and muscle from control, respectively. Of the differentially expressed genes, 119 were shared by all three tissues. Tissue-overlapping transcripts and the pathways and functions they defined may underline the primary response of fish to thermostressing. High temperature may cause directly protein mis-folding and hypoxia. Cells suffering thermostress either survive hypoxia or are eliminated by immune system. High temperature may have interacted indirectly through HSPs with HIF-1 and JAKs/STATs signaling pathways. The former up-regulates the expression of hypoxia inducible genes while the later up-regulates the expression of genes associating with insulin and NOD-like receptors signaling pathways. Insulin pathway functions to sustain homeostasis of sugar and lipids, aiding to survive cells, while NOD-like receptors signaling pathway strengthens immune, apoptotic and inflammatory responses, helping to survive cells. These understandings may help improve our culturing practice, the culture performance of half-smooth tongue sole and its breeding.

Effects of aging on the fraction distribution and bioavailability of selenium in three different soils.

Aging refers to the processes by which the mobility and bioavailability of metals in soil decline with time. Although long-term aging is a key process that needs to be considered in risk assessment of metals, few investigations has been attempted to determine whether and how residence time influences the selenium (Se) fractions and bioavailability in soil. In this study, the fractions of Se in soils was evaluated, and bioavailability were assessed by measuring Se concentration in pak choi (Brassica chinensis L.). Results showed that the change of soil available Se in all tested soils divided into two phases: rapid decrease at the initial time (42 d) and slow decline thereafter. The second-order equation could describe the decrease processes of available Se in tested soils during the entire incubation time (R(2) > 0.99), while parabolic diffusion equation had less goodness of fit. Those results indicated that Se aging was controlled not only by diffusion process but also by other processes such as nucleation/precipitation, adsorption/desorption with soil component, occlusion by organic matter and reduction reaction. Soil available Se fractions tended to transform to more stable fractions during aging. The changes of Se concentration in pak choi were consistent with the variation in soil available Se content. In addition, 21 d could be reference for the time of Se aging reaching stabilization in krasnozems and fluvo-aquic soil, and 30 d for black soil. Results could provide theoretical basis to formulate environmental quality criterion and choose the equilibrium time before implementing a pot experiment in Se-spiked soils.